Fig. 8.

EhC2A shRNA protein knockdown transfectants modulate URE3 motif-regulated transcripts. mRNA levels of URE3-BP, acyl-CoA synthetase, and membrane protein from E. histolytica trophozoites transfected with EhC2A (363–391) (shRNA) or EhC2A (363–391 scrambled) (control) as measured by qRT-PCR (mean ± standard error). Average results for three biological replicates are expressed as the percentages of the EhC2A (363–391 scrambled) level (control), standardized for each transcript with plasmid control mRNA levels and normalized to the actin level. Statistical analysis was performed using Student's t test. **, P < 0.05.