Fig. 1.

Biolistic transformation and characteristics of mtDNA transformants. (A) Transformants on bombarded plates. A YPD culture of 20 ml (30°C, 200 rpm, 24 h) of C. glabrata Δura3 Δarg8 cells was concentrated, washed with 1 mol·liter⁻¹ sorbitol, spread on MM plates, and transformed with 5 μl of 2-μg·μl⁻¹ pUC19-atp6::ARG8m using a particle gun. Plates were incubated at 30°C for 72 h until transformants appeared. (B) mtDNA transformants on MM and YPG plates. Overnight cultures (10 μl) of C. glabrata Δura3 Δarg8 (CK) and mtDNA transformants were spread on YPD and YPG and cultured at 30°C for 72 h. mtDNA transformants yielded colonies similar to those of CK. mtDNA transformants also grew on YPG, although more weakly. (C) mtDNA transformants lose the ability to grow on MM. After aerobic growth in YPD, some colonies lost the ability to grow on MM while retaining the ability to grow on SM. The ratio of MM to SM colonies was 32.5% based on the analysis of a total of 500 colonies.