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. 2010 Apr 21;30(16):5713–5723. doi: 10.1523/JNEUROSCI.1001-10.2010

Figure 7.

Figure 7.

Retrograde tracing from IPAC labels VTA LepRb neurons. The retrograde tracer FG was stereotaxically injected into the IPAC of LepRbEGFP animals to determine the potential projection of VTA LeRb neurons to the IPAC by colocalization of FG and EGFP immunoreactivity. A, B, Schematic diagram (A) and fluorescent image (B; red, FG; green, EGFP) of the IPAC injection site in a representative animal. C, Distribution of FG- and EGFP-IR neurons in the VTA. Images below are digital zooms of the boxed areas showing (top to bottom) merged images, FG immunoreactivity, and EGFP immunoreactivity. Arrows indicate colocalized neurons. Red asterisks indicate the BLA. Scale bars: B, C, 200 μm; insets, 20 μm. IP, Interpeduncular nucleus; ml, medial lemniscus; 3v, third ventricle.