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. 2010 Apr 21;30(16):5713–5723. doi: 10.1523/JNEUROSCI.1001-10.2010

Figure 9.

Figure 9.

Identification of CART-expressing CeA neurons as targets of leptin action. A, Schematic diagram showing the generation of LepRb-WGA mice. Leprcre mice were crossed with iZ/WAP transgenic mice to mediate the expression of the trans-synaptic tracer WGA in LepRb neurons. IRES, Internal ribosome entry site; AP, alkaline phosphatase; pA, polyadenylation site. B, WGA immunoreactivity in the hypothalamus and amygdala of a LepRb-WGA mouse. opt, optic tract. Inset, Higher-magnification image showing WGA immunoreactivity in the CeA. Scale bars, 200 μm. C–E, WGA-IR (C, green), CART-IR (D, red), and merged (E) confocal images from the CeA of a LepRb-WGA mouse. Arrows indicate colocalized neurons. Scale bars are as indicated. F, Wild-type (WT) and leptin-deficient Lepob/ob (ob/ob) mice were treated with leptin (5 mg/kg, i.p.) or vehicle 12 h for 24 h before dissection and mRNA extraction from the CeA. Expression of Cart mRNA was quantified by quantitative PCR and is plotted as mean ± SEM. n = 9–10 per group; *p < 0.05, compared with WT by ANOVA.