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. Author manuscript; available in PMC: 2011 Apr 23.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Mar 31;395(1):136–140. doi: 10.1016/j.bbrc.2010.03.156

Figure 1. FLAG-CaSR and myc-p24A interact in the early secretory pathway.

Figure 1

HEK293 cells transfected with FLAG-CaSR or FLAG-CaSR∧F plus control vector (pcDNA) or myc-p24A were immunoprecipitated (after 24 hrs) with anti-myc (left panel) or anti-FLAG (right panel) antibodies and processed as described in Methods. Blots were probed with anti-CaSR antibody (upper portion) and anti-myc antibody conjugated with HRP. Dotted lines indicate different sections of the same blot processed separately with the indicated antibodies.