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. 2010 Feb 24;30(8):2967–2978. doi: 10.1523/JNEUROSCI.5552-09.2010

Figure 7.

Figure 7.

Depletion of cytosolic NAD+ with NADase kills neurons independent of PARP-1 activation. A, NADase transfection (10, 30, 100 μg/ml) produced a dose-dependent decrease in neuronal NAD+ content in both wild-type and PARP-1−/− neurons, as measured 3 h after BioPORTER transfection (BP). This decrease was blocked by the NADase inhibitor NAM (200 μm) and not observed in cultures treated with the BioPORTER vehicle alone (far right bar). B, NADase transfection also produced a dose-dependent neuronal death in both wild-type and PARP-1−/− neurons, evaluated 24 h later. This decrease was blocked by the NADase inhibitor NAM (200 μm) and not observed in cultures treated with the BioPORTER vehicle alone. C, Neuron death caused by NADase transfection (30 μg/ml) was significantly prevented by exogenous NAD+ treatment (2.5, 5, and 10 mm) in both wild-type and PARP-1−/− neurons. Error bars indicate SEM. *p < 0.01, **p < 0.001 (in A and B, versus control; in C, versus NADase alone).