Table 1.

Effects of PARP-1 activation on glycolytic intermediates upstream of GAPDH

Condition	DHAP (pmol/mg)	GAP (pmol/mg)	FBP (pmol/mg)
Control	2.24 ± 0.74	0.93 ± 0.26	1.64 ± 0.97
MNNG	11.18 ± 2.57#	2.37 ± 0.62#	8.52 ± 1.69#
MNNG + PJ34	2.28 ± 0.96**	1.06 ± 0.23*	2.22 ± 1.29**
MNNG + DPQ	2.58 ± 0.84**	1.20 ± 0.34	3.19 ± 1.15**
MNNG + NAD	1.52 ± 0.90**	1.13 ± 0.22*	3.39 ± 1.29**
IA	9.83 ± 2.06#	3.10 ± 1.14#	6.93 ± 0.73#

Mouse cortical neurons were exposed to 75 μm MNNG for 30 min. PJ34 (200 nm) and DPQ (25 μm) were added with MNNG, and NAD⁺ (5 mm) was added after MNNG washout. Levels of DHAP, GAP, and FBP were measured at 30 min after MNNG washout. Iodoacetate (IA) (250 μm) was used as a positive control for GAPDH inhibition. Data are means ± SEM (n = 3).

^#p < 0.01 versus control; *p < 0.05, **p < 0.01 versus MNNG.