Table 1.
Inhibition of HIV-1 by β-Chemokines in the PBMC Assay
| |
IC80(ng/ml) in PBMCsa |
||||||
|---|---|---|---|---|---|---|---|
| Recombinant protein | Bal R5 | SF162 R5 | WITO R5 | CH040 R5 | CH058 R5 | CH077 R5 | WEAU R5X4 |
| MIP-1α | 400 | >1250 | 120 | 126 | >1250 | >1250 | >1250 |
| MIP-1α/LD78β | 13 | 43 | 10 | 7 | 18 | 109 | >1250 |
| MIP-1β | >1250 | >1250 | 1160 | 615 | >1250 | >1250 | >1250 |
| RANTES | 66 | 171 | 36 | 37 | 75 | >1250 | >1250 |
| IFN-γ | >5000 | ND | ND | ND | ND | ND | >5000 |
| MDC | >1250 | ND | >1250 | ND | ND | ND | >1250 |
a
Chemokines and IFN-γ were assayed in PBMCs against PBMC-grown NL-LucR.T2A-Env.ecto reporter HIV-1 expressing the indicated envs. Values are the concentration at which RLU were reduced 80% compared to virus control wells after subtraction of background RLU in cell control wells. ND, inhibition experiments were not done with these viruses.