FIG. 1.

Sequence variation in primer binding sites for ASPCR assay. Variations in the primer binding site sequences for the ASPCR assay necessitated creating nine different primer sets for the 103 position and eight different primer sets for the 181 position of reverse transcriptase with the wild-type primer binding site polymorphisms shown above. The asterisk (*) is over the site of interest where a nucleotide change confers drug resistance at both positions 103 and 181. The reverse wild-type primer as shown has an intentional mismatch at the (–2) position to enhance specificity. (n) represents the number of samples within this data set that matches each primer set.