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. Author manuscript; available in PMC: 2010 May 4.
Published in final edited form as: Nature. 2008 Mar 12;452(7188):773–776. doi: 10.1038/nature06764

Figure 1. Lack of IL-17 production in SEB-stimulated PBMCs from HIES patients.

Figure 1

a, PBMCs from cohorts of HIES patients with stat3 mutations (red circles), HIES-like patients (blue circles) and healthy individuals (green circles) were stimulated overnight with SEB in the presence of brefeldin A and stained as described in Methods. The frequency of memory CD4 T cells (gated as described in Methods) that produced IFN-γ, IL-2, TNF or IL-17 was then assessed. b, Memory CD4 T cells that produced IFN-γ were analysed for their ability to co-produce IL-2 and TNF by using SPICE (Simplified Presentation of Incredibly Complex Evaluations) as described in Methods. Statistical significance was determined with the Mann–Whitney test. Significant P values are shown in red, and median values with horizontal bars.