Fig. 8.

Actions of flumazenil and baclofen, but not naloxone, against stress-enhanced ethanol withdrawal anxiety. Rats were exposed to control diet (CD), restraint stressed twice (6 days apart; 45 min each session), and then given 4.5% ED for 5 days before social interaction (SI) testing. Thirty minutes before each stress (10 min for flumazenil), rats were treated with either vehicle, flumazenil (5 mg/kg), naloxone (20 mg/kg), or baclofen (2.5 mg/kg), respectively. Social interaction testing was carried out 5 h after ethanol exposure was stopped. Bars represent the means ± SEM. Groups with different letters are significantly different from each other (Tukey’s protected t-tests).