Table 1.
Summary of the extraction results from 900 colorectal cancer formalin-fixed paraffin-embedded tissue cores
| DNA | RNA | |
|---|---|---|
| Quantity Check | ||
| Mean Yield per Core, µg | 9.31 | 8.12 |
| Range of Yield per Core, µg | 3 – 141 | 1 – 192 |
| Quality Check | ||
| Gel Imaging Fragment Size | 15–30kb | ≥ 200nt |
| 260/280 Ratio | 1.79 – 1.95 | 1.90 – 2.11 |
| PCR Amplification | successful for 99% of samples3 | — |
1
Measured by Invitrogen’s PicoGreen assay, quantifying double-strand nucleic acids
2
Measured by optical density Nanodrop, quantifying single-strand nucleic acids
3
Using specific primers targeting DNA fragments between 35–500 bp