Figure 3.
Coupling stoichiometry of 2'-O-methyl oligoribonucleotide affects the potency of dextran-conjugated antisense reagents. (a) Schematic structures of dextran-(as-2'OMelin-4)8, Rhdextran-(as-2'OMelin-4)4 and Rhdextran-(as-2'OMelin-4)1. The heavy blue line, wavy green line and the red dot symbolize dextran, 2'-O-methyl oligoribonucleotide and rhodamine, respectively. (b) Dose response of dextran-(as-2'OMelin-4)8, Rhdextran-(as-2'OMelin-4)4, and Rhdextran-(as-2'OMelin-4)1 in causing egg laying defectives. Error bars are standard errors of two independent experiments. Each time we scored at least 50 worms labelled with an antisense reagent.