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. Author manuscript; available in PMC: 2010 May 4.
Published in final edited form as: Nat Chem Biol. 2009 Jun;5(6):421–427. doi: 10.1038/nchembio.168

Figure 3.

Figure 3

G15 antagonism of intracellular calcium mobilization by GPR30. (a) The effect of G15 on the subsequent mobilization of calcium by G1, E2 or ATP was evaluated using indo1-AM-loaded SKBr3 cells. G15 (1 μM, red line) or vehicle (ethanol, black line) was added at 20 sec. (first arrow). G-1 (200 nM), 17β-estradiol (E2, 100 nM) or ATP (1μM, a purinergic receptor control) was added at 80 sec. (second arrow). (b) Dose response profile of G-1-stimulated SKBr3 cells to increasing concentrations of G15. (c) Dose response profile of 17β-estradiol-stimulated SKBr3 cells to increasing concentrations of G15. In panels b and c, G-1 and 17β-estradiol were used at 100 nM and 30 nM, respectively, concentrations that yield approximately the half-maximal calcium response for each ligand (approximately 25% that of the full ATP response). Data in panel a are representative of at least three independent experiments. Data in panels b and c represent the mean ± s.e.m. from at least three separate experiments.