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. Author manuscript; available in PMC: 2010 May 4.
Published in final edited form as: Nat Chem Biol. 2009 Jun;5(6):421–427. doi: 10.1038/nchembio.168

Figure 5.

Figure 5

Effects of G15 on physiological responses mediated by GPR30. Epithelial uterine cell proliferation was assessed in the presence of E2 or E2 + G15 (a) or in the presence of G-1, G15, or G-1 + G15 (b) in ovariectomized female C57Bl6 mice. Compounds (amount in parentheses indicates nmol/mouse). Proliferation of uterine epithelium was quantitated by immunofluorescence using anti-Ki-67 antibody. (c) Immobility in adult male ICR mice was assessed as an indicator of depression. Mice were suspended from the tip of the tail and the total amount of time each animal remained immobile during a 6-min period was recorded. Compounds (amount in parentheses indicates nmol/mouse; desipramine and soluble E2 were used at 10mg/kg and 5 mg/kg respectively) were administered intraperitoneally. Each group consisted of 10-12 animals. For all panels, results are expressed as mean ± s.e.m., and statistical significance (P<0.05) was assessed by student’s t test: *, significantly different than sham; **, significantly different than E2 or G-1, respectively.