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. 2010 May 4;5(5):e10474. doi: 10.1371/journal.pone.0010474

Figure 1. Transposon-inactivated region in the bacterial chromosome.

Figure 1

A Gene organization of 31G12 mutant associated with induction of macrophage apoptosis. The operon contains seven genes, including four genes (Rv3657c–Rv3660c) that resemble a pilus assembly proteins, type II/IV secretion system proteins, as well as tight adherence genes of TadZ, TadA-C, respectively; Rv3656c transmembrane protein and Rv3654c, Rv3655c genes that encode secreted proteins. The arrow indicates the gene disrupted by the insertion of Tn5367 transposon (TN). B M. tuberculosis gene expression following 30 min exposure to macrophages or 3 h infection. Total RNAs from broth-grown bacteria, as well as from intracellular- and macrophage-exposed bacteria, were used to determine the copy numbers of cDNAs for target and reference genes. Data were analyzed on Ct values basis for each sample and normalized with an internal housekeeping gene control, 16S rRNA.