Table 4. Effect of depletion of target molecules on parasite growth, viability, mitochondrial potential.
Gene | Relative live cell numbera | % dead cells | Relative mito ψb |
TOPIBS | n.d. | 35 | 1.10 |
0.16 | 28 | 1.34 | |
TOPIBL | n.d. | 72 | 1.33 |
0.21 | 28 | 0.93 | |
TOPIImt | 0.08 | 44 | 0.44 |
0.51 | 19 | 0.33 | |
PFTB | 0.36 | 14 | 0.72 |
0.3 | 17 | 0.68 | |
PEX19 | n.d. | 64 | 0.47 |
0.26 | 61 | 0.52 | |
KREPA3 | 0.05 | 37 | 0.19 |
0.15 | 19 | 0.41 | |
NOPP44/46 | 0.04 | 26 | 0.34 |
0.12 | 17 | 0.53 |
Defined as the cumulative reduction in live cell number relative to uninduced controls at day of assay. TOP1BS, TOP1BL, and PEX19 knockdowns were assayed on day 2 post induction of RNAi, while PFTB and NOPP44/46 knockdowns were assayed on day 3 and TOPIImt on day 6. KREPA3 was assayed five days after removal of Tet.
as compared to control cells in the same experiment.