Table 1.
Candidate qPCR reference genes tested in this study with the putative function of the proteins they encode and accession numbers of the produced epaulette shark gene sequences.
| Symbol | Gene (homolog) name | Function | Genbank accession |
|---|---|---|---|
| actc1 | alpha-actin, cardiac muscle 1 | Structural protein | GU983379 |
| dnaja2 | dnaJ subfamily A 2 (Hsp40) | Chaperone | GQ152302 |
| eef1b | eukaryotic translation elongation factor 1 beta | Protein synthesis | GU983380 |
| hprt | hypoxanthine phosphoribosyltransferase 1 | Purine nucleotide synthesis | GQ152303 |
| mrip | myosin phosphatase-Rho interacting protein | Component of the myosin phosphatase complex | GQ152304 |
| polr2f | polymerase (RNA) II (DNA directed) polypeptide F | Component of RNA polymerase II | GQ152305 |
| rpl6 | 60S ribosomal protein L6 | Component of the large 60S ribosomal subunit | GU983381 |
| tubb2 | tubulin beta 2 | Structural protein | * |
| ubq | ubiquitin | Protein degradation | GQ152306 |
* qPCR primer designed directly from the tubb2 alignment of following vertebrate species: human ENST00000340384, chicken ENSGALT00000022476, frog ENSXETT00000047287, stickleback ENSGACT00000016810, zebrafish BC062827.1