Table 4.
Primers used in this study.
| Gene | Universal primers Forward/Reverse |
qPCR primers Forward/Reverse |
amplicon (bp) | E |
|---|---|---|---|---|
| actc1 | - | CACCTTCCAGCAGATGTGGAT TACTGGGCGGGACTGAGATT |
101 | 0.93 |
| dnaja2 | YRTGCGBRTYATGATHAGACA GACMSAVCCTGGYTCRATDACTTT |
AAGAAGGTGGTGAAAGAAGTGAAGA CAAAGTGCCTCAACAAGACCA |
217 | 0.71 |
| eef1b | - | GCTGTGGATGATGACGACGA GCACCCCAAAGTAAGCCATC |
247 | 0.90 |
| hprt | GCTGAYCTVYTDGAYTACATCAA GGGCRTADCCYACMACRAAYTTGTC | TCAATCCCAATGACGGTAGACTT AGTCTGCATTGTTTTACCCGTATC |
161 | 0.83 |
| mrip | GRGTRGAGAGYGGYTACTT CARTCRAAKGTYTTBGAGCG |
ACAGTACCGCAGGGCAAAGT CCAATGCTTCTTCCACTGACC |
126 | 0.92 |
| polr2f | ATGTCNGAYAACGARGAYAAYTT YTCRTCMMMNCCCCAGTC |
TGATGGAGATGACTTTGACGATG ACACGGGCTCGCTCATACTT |
170 | 0.84 |
| rpl6 | - | CACCAGGAGGGCGAGATCT TGGTCATCCTTCCGCTGTTC | 71 | 0.96 |
| tubb2 | * | CACCTTCATTGGCAACAGCAC GGCCTCTGTGAACTCCATCTC |
98 | 0.91 |
| ubq | CYGGSAAGACCATCACCCT GGTSGACTCYTTCTGGATGTTGTA |
CTGGCAAACAGCTGGAAGA ATCTGCATGCCACCTCTCA |
99 | 1.01 |
For part of the genes universal primers were used to acquire novel Epaulette shark sequences. The amplicon length and PCR efficiency (E) of qPCR primers is displayed.
* qPCR primer designed directly from the tubb2 alignment, see table 1