Figure 8. dsx^GAL4 is expressed in a subset of glial cells in the CNS.

(A) dsx^GAL4 is expressed in cells with glial morphology (arrows) that are associated with the cortical surface of the brain in a female at 48 h APF, as shown using UAS-mCD8::GFP membrane-bound GFP reporter. Shown here is a projection of the anterior brain, revealing cells across the medial optic cleft and suboesophageal ganglion (arrowhead). Expression is also seen in some samples at the cortical surface of the lateral horn (barbed arrowheads). Neuropile is counterstained with DN-Cadherin (DN-Cad). (B) dsx^GAL4 expression in the anterior brain of a 0 d adult male using the UAS-Stinger nuclear GFP reporter. Costaining for the neuronal marker ELAV (red) and the glial marker REPO (blue) reveals overlap with dsx^GAL4 in a subset of neurons and glia. In the region of the anterior Dorsal Neurons (aDN, detailed in panel C), we observe overlap with ELAV but not REPO. However, in the anteroventral optic cleft, we observe overlap with REPO but not ELAV, confirming the glial cell type of the Suboesophageal Lateral Glia (SLG; detailed in panel D). (C) The aDNs (green) are positive for ELAV (red) but not REPO (blue). (D) The SLG in the anteroventral optic cleft are positive for REPO but not ELAV.