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. 2010 May 4;4(5):e671. doi: 10.1371/journal.pntd.0000671

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A) Uninfected head tissues of Cx. quinquefasciatus stained with AlexaFluor 594 (red). White arrow depicts non-specific staining of debris. B) Head tissues of CxFV Izabal-infected Cx. quinquefasciatus, harvested 7 DPI. CxFV Izabal stained with AlexaFluor 594. C) Head tissues of WNV-infected Cx. quinquefasciatus, harvested 9 DPI. WNV stained with AlexaFluor 488 (green). D) Co-infected head tissues of Cx. quinquefasciatus. Mosquito inoculated simultaneously with CxFV Izabal and WNV, harvested 9 DPI. CxFV Izabal stained with AlexaFluor 594 (red) and WNV with Alexa 488 (green). White arrow denotes non-specific staining of debris.