Table 1. qRT-PCR primers and probes used for detection and quantification of CxFV Izabal RNA.

Target	Primer name	5′ to 3′ sequence	Tm	Nucleotides different from CxFV from Japan (NC_008604)	Nucleotides different from CFAV (NC_001564)
E	E92F	CGCCGAACGGACTTCTTG	59	0	4
	E151R	TCCATTGGCCGCCATATATC	59	0	4
	E112Probe	TTCCGCACTGGAGCAGCCG	70	2	8
E	E232F	GGTGAGATCAACGGCAAAGAA	59	2	7
	E295R	CAGTTCCCCATCCACGATTG	59	0	4
	E295Probe	CGTTTGCTCAACCCAGCCCT	68	1	4
NS5	NS5372F	CCACACCAGTCTTCGGTACATC	58	2	8
	NS5438R	CGGTTCGGTAGGTTGCAAGT	59	3	11
	NS5395Probe	CTGCTGCGTCAAAAACGCGCAA	69	2	8
NS5	NS5618F	GGCTCACGCCCAGATGTG	60	3	3
	NS5688R	TGATGGCGGCGAATCC	59	2	5
	NS5638Probe	CGTTGTACTACTTCCATCGGAGAGATCTGCG	69	5	8