Figure 2.

IHC analysis at P60 of visual arrestin expression in mice retinas from control (WT) and visual arrestin knockouts (Arr1^−/−, Arr4^−/−, Arr-DKO). Retinal frozen sections of WT (A), Arr1^−/− (B), Arr4^−/−(C), and Arr-DKO (D) were processed with Arr1 (mAb D9F2) and Arr4 (pAb mCAR-LUMIj) antibodies, followed by the appropriate secondary antibodies. Immunoreactive Arr1 is red (Texas red) and Arr4 is green (fluorescein). Dual immunolocalization of Arr1 and Arr4 is yellow. Nuclei are stained blue with DAPI. IS, inner segment; OS, outer segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer. Original magnification, 40×. Scale bar, 20 μm.