Fig. 6.

GFAP immunoreactivity in the spinal cord of EphA4+/- and EphA4−/− mice 14 days after SCI. (A, B) Lower magnification sagittal view of GFAP expression at the dorsal hemisection injury site (indicated by a white arrow) in a representative EphA4+/+ (A) or EphA4−/− (B) mouse. Note a much clearer border between largely GFAP-positive cells and the largely GFAP-negative lesion core in both (A) and (B), as compared to Fig. 5A and B. (C, D) High magnification view of the dotted boxes in (A) and (B) respectively, showing alignment of GFAP-positive cells/processes parallel to the lesion edge in both EphA4+/+ (C) and EphA4−/− (D) mice. (E, F) Higher magnification view of the dashed boxes in (A) and (B) respectively, showing the largely GFAP-negative lesion core. Lesion edges are demarcated with dash-dot lines. Note the similar pattern of overall low level of GFAP expression in the lesion core that contains few GFAP-positive processes in control (E) and mutant (F) mice. (G, H) Higher magnification images of GFAP-positive cells ~400 μm from the lesion core, showing similar morphology between EphA4+/+ (G) and EphA4−/− (H) mice. Scale bar = 300 μm (A, B); 60 μm (C-H).