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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Exp Neurol. 2009 Sep 8;223(2):304–310. doi: 10.1016/j.expneurol.2009.08.031

Figure 3. Schematic representation of a FP-based tau assembly assay and the kinetics of tau fibrillization.

Figure 3

A FP assay was utilized to monitor tau assembly in which Alexa Red-labeled K18PL was mixed at a 1:62.5 molar ratio with unlabeled K18PL. A) Upon addition of heparin and initiation of tau assembly, Alexa Red-labeled K18PL is incorporated into tau multimers, thereby decreasing the rotational freedom of the fluorescent label with a resulting increase in FP signal. B) FP signal as a function of time in a K18PL fibrillization reaction.