Figure 4.

CLL cells from ZAP-70⁻ patients reduce T-cell migration towards CXCL12. Purified T cells from ZAP-70⁻ (n= 10) and ZAP-70⁺ CLL patients (n=9) were cultured in complete medium alone (pT cultures) or at a 1:4 ratio with autologous purified CLL cells (pT+pCLL cultures). Chemotaxis assays towards CXCL12 (1 μg/mL) were performed with freshly purified cells (A) and 48 h cultured cells (B). The migration of CD3⁺ cells was calculated by taking the migration index of T cells in pT+pCLL cultures as 100%. Bars represent the mean values±SEM for the CD3⁺ migration. NS: not significant, *P=0.001, Wilcoxon’s signed rank test. The lower panels show the migration indices of pT+pCLL and pT from 48 h cultured cells of each patient, *P<0.01, Wilcoxon’s signed rank test.