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. 2009 Dec 16;95(5):776–784. doi: 10.3324/haematol.2009.015628

Figure 4.

Figure 4.

Stable knockdown of HIF-1α and HIF-2α in LP-1 cells. (A) RNA interference was used to knock down endogenous HIF-1α or HIF-2α expression in LP-1 cells, and down-regulation of HIF protein confirmed by western immunoblotting. (B) Levels of CXCL12 mRNA expression were measured in the HIF knockdowns in response to normoxic (white bars) or hypoxic (black bars) culture. Columns, mean (n=3); bars, SEM. *P<0.05, **P<0.001, compared to vector control. (C) Levels of CXCL12 protein were measured in conditioned media from the HIF knockdowns following 72 h of normoxic (white bars) or hypoxic (black bars) culture. Columns, mean (n=3); bars, SEM. *P<0.05, compared to vector control.