Fig. 4.

Effects of myricetin on UVB-induced HIF-1α expression in SKH-1 hairless mice. (A and B) Myricetin inhibits UVB-induced HIF-1α expression in SKH-1 hairless mice. (a) Vehicle-treated controls, (b) UVB-irradiated (0.18 J/cm²) mice and mice treated with UVB plus (c) 8 nmol of myricetin or (d) 20 nmol of myricetin. The mice were treated as described in Figure 1B. Serial sections were mounted on silane-coated slides and immunostained for HIF-1α as described in Materials and Methods. The photos are representative of results from five or six tissue samples. HIF-1α appears brown. HIF-1α or β-actin expression was assessed by western blotting as described in Materials and Methods. Each band was densitometrically quantified by image analysis. The results are shown as means ± SEMs (n = 3). The pound (#) symbol indicates a significant difference (P < 0.05) between the control and UVB-irradiated groups, whereas the asterisk (*) indicates a significant difference (P < 0.05) between the UVB-irradiated/myricetin-treated and UVB-only irradiated groups.