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. 2009 Dec 11;31(5):911–917. doi: 10.1093/carcin/bgp221

Fig. 6.

Fig. 6.

Effect of myricetin on UVB-induced PI-3 kinase activity in SKH-1 hairless mice. (A) Myricetin inhibits UVB-induced PI-3 kinase activity in a dose-dependent manner. To assay for PI-3 kinase activity, dorsal skin lysates were prepared from the epidermis and subjected to immunoprecipitation and a PI-3 kinase assay as described in Materials and Methods. The results are shown as means ± SEMs (n = 3). The pound (##) symbols indicate a significant difference (P < 0.01) between the control and UVB-irradiated groups; the asterisk (*) indicates a significant difference (P < 0.05) between the UVB-irradiated/myricetin-treated and UVB-irradiated groups. (B) Myricetin binds PI-3 kinase directly in mouse skin lysates. In vivo myricetin binding was confirmed by immunoblotting using an antibody against subunit p110: lane 1 (input control), whole lysate from mouse dorsal skin; lane 2 (control), mouse dorsal skin lysate precipitated with Sepharose 4B beads (see Materials and Methods) and lane 3, whole-cell lysate from mouse dorsal skin precipitated by myricetin–Sepharose 4B affinity beads (see Materials and Methods). (C) Simplified view of the proposed anti-angiogenic mechanism of myricetin.