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. 2010 Jan 20;31(5):785–793. doi: 10.1093/carcin/bgq014

Fig. 2.

Fig. 2.

Plk1 is required for the PTP inhibitor-induced mitotic progression after Cr(VI) exposure. (A) HLF cells were coincubated with or without the Plk1 inhibitor, GW843682X (GlaxoSmithKline R&D), SOV and Cr(VI) for 24 h, washed, collected and fixed. Mitotic cells were determined by histone H3 phosphorylation as described in the legend of Figure 1 and are the mean of four independent experiments. (B) Quantitative data of above experiment. Data are the means ± standard error of four independent experiments. Asterisk indicates a statistically significant difference from the respective control at P < 0.05. (C) HLF cells were transiently transfected with the kinase-inactive K82R mutant. Cells were treated with the respective agents for 24 h at 48 h posttransfection and then washed, collected and fixed. Mitotic cells were determined as described in the legend of Figure 1 and are representative of one experiment. (D) Quantitative data of above experiment.