FIG. 1.

Representative images of gels from a sham-operated rat (left column) and a spinal cord-injured rat (right column), which were mapped according to isoelectric point (pI) and molecular weight (MW). Thirty-eight gel spots were significantly different between the injured and sham spinal cords with respect to protein abundance, phosphorylation, or both (colored numbers and arrows). SYPRO Ruby Stain (bottom row): 19 gel spots were identified only in the injured samples (orange; see Table 1); 15 gel spots were identified in both groups but protein abundance either increased (dark blue) or decreased (light blue) after injury (see Table 2). The insets on the SYPRO Ruby-stained gels provide higher magnification of corresponding regions of the sham and injured samples, illustrating several gel spots that were identified only after the injury. The black numbers and arrows on the SYPRO Ruby-stained gels indicate spots that did not differ in abundance between the injured and sham cords; however, the phosphorylation state of these spots changed with injury and is included for comparison with the Pro-Q Diamond-stained gels. Pro-Q Diamond Stain (top row): five phosphorylated spots were identified in the injured cords only (green; see Table 3A); four phosphorylated spots were identified in both groups of rats but phosphorylation level either increased (brown) or decreased (tan) after injury (see Table 3B). Ovalbumin (red asterisks) is the internal standard.