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Experimental Protocol
| Infusion rate | PMA 15 μM (50 μg/kg) | PMA 50 μM (200 μg/kg) | Vehicle | |
|---|---|---|---|---|
| − 60 min | 480 μL/h | n = 6 | n = 6 | n = 6 |
| MCA occlusion | ||||
| +30 min | 640 μL/h | n = 6 | n = 6 | |
| +60 min | 720 μL/h | n = 6 | n = 6 | |
| Reperfusion | ||||
| +2 h | ||||
| Sacrifice | n = 6 | n = 18 | n = 18 | |
| +4 h |
MCA, middle cerebral artery; PMA, phorbol 12-myristate 13-acetate.
