FIG. 5.

Effect of buprenorphine on white matter spare tissue at 14 DPI. Histological studies were performed using luxol staining and cresyl violet counterstaining. White matter spare tissue was evaluated at the lesion epicenter in injured animals treated and untreated with buprenorphine at 14 DPI. The area of spare tissue was morphometrically analyzed as density ×area (A). The values presented are the mean ± SEM. Luxol and cresyl violet stained representative segment of a lesion epicenter of an untreated (B) and a treated (C) injured animal at 14 DPI are shown. No significant difference was observed in any of the groups compared at any of the segments evaluated (Student t test, p > 0.05, n = 6). Scale bar, 100 μm.