Table 1.
ELISA | Parameters in four parameter curve fit |
|||||
---|---|---|---|---|---|---|
n | Min (abs) | Max (abs) | EC (50) | Slope | Intra-assay CV (%) | |
Synaptophism | 9 | 0.02 ± 0.00 | 1.46 ± 0.23 | 17.2 ± 2.9 | 1.49 ± 0.12 | 8.4 |
SNAP-25 | 8 | 0.01 ± 0.00 | 2.53 ± 0.12 | 137.5 ± 22.9 | 1.08 ± 0.08 | 8.7 |
PSD-95 | 8 | 0.01 ± 0.00 | 1.05 ± 0.09 | 187.7 ± 16.7 | 1.46 ± 0.14 | 7.6 |
GFAP | 7 | 0.04 ± 0.01 | 2.46 ± 0.07 | 5.0 ± 0.7 | 1.05 ± 0.04 | 6.0 |
Data are shown as mean ± SEM
n number of statndard curve trials, MIN minimum absorbance in four-paramter fit, MAX maximum absorbance in four-parameter fit, EC50 concentration of extract where 50% of maximum binding (absorbance) is observed, slope the slope of the four-parameter fit, intra assay CV intra assay coefficient of variation