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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Exp Brain Res. 2010 Feb 19;201(4):885–893. doi: 10.1007/s00221-010-2182-x

Table 1.

Synaptic marker ELISA parameters

ELISA Parameters in four parameter curve fit
n Min (abs) Max (abs) EC (50) Slope Intra-assay CV (%)
Synaptophism 9 0.02 ± 0.00 1.46 ± 0.23 17.2 ± 2.9 1.49 ± 0.12 8.4
SNAP-25 8 0.01 ± 0.00 2.53 ± 0.12 137.5 ± 22.9 1.08 ± 0.08 8.7
PSD-95 8 0.01 ± 0.00 1.05 ± 0.09 187.7 ± 16.7 1.46 ± 0.14 7.6
GFAP 7 0.04 ± 0.01 2.46 ± 0.07 5.0 ± 0.7 1.05 ± 0.04 6.0

Data are shown as mean ± SEM

n number of statndard curve trials, MIN minimum absorbance in four-paramter fit, MAX maximum absorbance in four-parameter fit, EC50 concentration of extract where 50% of maximum binding (absorbance) is observed, slope the slope of the four-parameter fit, intra assay CV intra assay coefficient of variation