APPENDIX 1.

Primers used to confirm microarray gene expression

Gene	Primer
Vcsa1:
Forward	5′-GAGGGTGTCAGAGGCCC-3′
Reverse	5′-GAGCAGTTAGCTGCCACTGATA-3′
Slo:
Forward	5′-TACTTCAATGACAATATCCTCACCCT-3′
Reverse	5′-ACCATAACAACCACCATCCCCTAAG-3′
Expi:
Forward	5′-TGTTCCAATGGCTGTGGTCA-3′
Reverse	5′-GGCCATCAGTCGTGCTTATGA-3′;
Krt1-18:
Forward	5′-CAGACCTTGGAGATTGACCTGG-3′;
Reverse	5′-TTGCTCCATCTGCACCCTGTA-3′;
Cav:
Forward	5′-ACCATCTTCGGCATCCCTATG-3′
Reverse	5′-AGGAAGCTCTTGATGCACGGT-3′;
Eef1a1:
Forward	5′-GTCAGAACGCAGGTGTTGTGAA-3′
Reverse	5′-GCCGGAATCTACGTGTCCAAT-3′;
Emp1:
Forward	5′-TCAAAGTGCATGCCCACCA-3′
Reverse	5′-GCGATGGAACATGTGCATCTC-3′ ‘;
RGD:1303126:
Forward	5′-TCTGACGGCAGGTCCTATGAGT-3′
Reverse	5′-TGGCCAGCATCTTTGCATC-3′.
Muc10:
Forward	5′-TCCCACCAAGGAGCAACATTAA-3′
Reverse	5′-GGATGTGGTTTTGGCTGGAAG-3′.
Alas2:
Forward	5′-ACCTCCCCTGCTGATTCAGAAT-3′
Reverse	5′-ACGGTATGTGTGGTCCTGCTTC-3′.
S100a9:
Forward	5′-ACCCTGAACAAGGCGGAATT-3′
Reverse	5′-TTTGTGTCCAGGTCCTCCATG-3′.
Pbsn:
Forward	5′-TGCTCACACTGGATGTGCTAGG-3′
Reverse	5′-TCCACGCTACTGGCAGCTAAGT-3′.
Rpl24:
Forward	5′-TCGAGCTGTGCAGTTTTAGTGG-3′
Reverse	5′-GCGGACTCACATTTGGCATTA-3′
Glyceraldehyde-3-phosphate
dehydrogenase
Forward	5′-GCCGCCTGCTTCACCACCTTCT-3′
Reverse	5′-GCATGGCCTTCCGTGTTCCTACC-3′