Table 2.
Splice junctions and insertions reported by SplitSeek with anchor length 22
| Oocyte 1 | Oocyte 2 | Oocyte 1+2 | |
|---|---|---|---|
| Number processed reads | 11,565,660 | 23,488,851 | 35,054,511 |
| Predicted splice junctions | 17,397 | 23,703 | 31,532 |
| Within chromosome | 16,205 (93.1%) | 21,495 (90.7%) | 27,957 (88.7%) |
| Within 1 Mb | 16,128 (92.7%) | 21,374 (90.2%) | 27,757 (88.0%) |
| Within 100 kb | 16,094 (92.5%) | 21,323 (90.0%) | 27,685 (87.8%) |
| Match to a RefSeq exon-exon boundarya | 14,264 (82.0%) | 18,139 (76.5%) | 23,235 (73.7%) |
| Expected false within 1 Mb (FDR) | 12.9 (8.0·10-4) | 17.6 (8.2·10-4) | 23.4 (8.4·10-4) |
| Expected false within 100 kb (FDR) | 1.3 (8.0·10-5) | 1.8 (8.2·10-5) | 2.3 (8.4·10-5) |
| Predicted insertions | 275 | 553 | 834 |
aEach of the exon boundaries located within 5 bp of predicted junction.