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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Comb Chem High Throughput Screen. 2010 Jan;13(1):75–87. doi: 10.2174/138620710790218258

Fig. (6). Fluorescence imaging of ErbB2 over-expressing cells for their binding to ErbB2ECDpositive clones.

Fig. (6)

The figure shows binding of representative clones 113 and 150 to MCF-7/ErbB2 and SK-BR-3 cells, which are known to over-express the ErbB2 receptor. High intensities of fluorescence are seen in both MCF-7/ErbB2 and SK-BR-3 cells in comparison to minimally expressive MCF-7 cells. Negative controls of wild-type β-lactamase (Wt-BLA) and unselected library clones did not show higher fluorescence in MCF-7/ErbB2 and SK-BR-3 cells. The precipitating green fluorescence is produced by the β-lactamase activity associated with clones.