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. Author manuscript; available in PMC: 2010 May 5.

Published in final edited form as: Phytochemistry. 2005 Jun;66(11):1211–1230. doi: 10.1016/j.phytochem.2005.03.031

Fig. 3 — Expression of the N. suaveolens BSMT. (a) BSMT transcript levels at different flower age: Plants were grown under LD cycle (16 h/8 h). Petals of N. suaveolens were harvested at 6 pm, in flower buds (day 1), at the day of flower opening (day 0) and at day 1 to 5 after flower opening. At day 5 flowers show obvious signs of senescence. (b) BSMT transcript levels at different time points during the day: Plants were grown under 16 h light and 8 h darkness (10 pm to 6 am). Petals of N. suaveolens were harvested at indicated time points during the day at the first and second day after flower opening. Black bars represent time of darkness, white bars represent time of illumination. (c) and (d) BSMT transcript levels under varied light regimes: Plants were grown under 16 h light and 8 h darkness (10 pm to 6 am) before they were shifted to continuous light conditions (LL for 4 days). Subjective night is represented by light gray shaded bars. Thereafter, light conditions were altered to 16 h light and 8 h darkness (10 to 6 pm darkness) for 3 days (LD). Black bars represent time of darkness, white bars represent time of illumination. Light was then switched off and plants were kept in complete darkness for 3 days (DD) (n.d. not determined). The subjective day is indicated by the dark gray shaded bar. Light conditions were then changed to 16 h light and 8 h darkness (4 pm to midnight) for 2 days (LD). Petals of N. suaveolens were harvested at indicated time points. RNA was extracted and RNA gels were run with 5 microgramm total RNA. The blots were hybridized with the N. suaveolens BSMT specific probe and rehybridized with 18 S rDNA probe. Relative transcript levels (normalized with rRNA data) were calculated, and the highest value was set as 100% (lowest panel).

Fig. 3 — Expression of the N. suaveolens BSMT. (a) BSMT transcript levels at different flower age: Plants were grown under LD cycle (16 h/8 h). Petals of N. suaveolens were harvested at 6 pm, in flower buds (day 1), at the day of flower opening (day 0) and at day 1 to 5 after flower opening. At day 5 flowers show obvious signs of senescence. (b) BSMT transcript levels at different time points during the day: Plants were grown under 16 h light and 8 h darkness (10 pm to 6 am). Petals of N. suaveolens were harvested at indicated time points during the day at the first and second day after flower opening. Black bars represent time of darkness, white bars represent time of illumination. (c) and (d) BSMT transcript levels under varied light regimes: Plants were grown under 16 h light and 8 h darkness (10 pm to 6 am) before they were shifted to continuous light conditions (LL for 4 days). Subjective night is represented by light gray shaded bars. Thereafter, light conditions were altered to 16 h light and 8 h darkness (10 to 6 pm darkness) for 3 days (LD). Black bars represent time of darkness, white bars represent time of illumination. Light was then switched off and plants were kept in complete darkness for 3 days (DD) (n.d. not determined). The subjective day is indicated by the dark gray shaded bar. Light conditions were then changed to 16 h light and 8 h darkness (4 pm to midnight) for 2 days (LD). Petals of N. suaveolens were harvested at indicated time points. RNA was extracted and RNA gels were run with 5 microgramm total RNA. The blots were hybridized with the N. suaveolens BSMT specific probe and rehybridized with 18 S rDNA probe. Relative transcript levels (normalized with rRNA data) were calculated, and the highest value was set as 100% (lowest panel).