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Determination of XWKK site size on polyU by stoichiometric binding. Titrations of (A) KWKK or (B) HWKK as a function of initial oligopeptide concentration (see legend above) at 3.4 mM Na+ and 25 °C (i.e., high affinity conditions). Intersection of the linear least square fits of the two regimes (dashed purple lines) is indicated by a light purple dotted line for clarity. The intersections for indicate a site size n = 5.3 ± 0.1 for KWKK and n = 5.4 ± 0.1 for HWKK.
