FIG. 5.

Grx1-deficient cells exhibit decreased NFκB transcriptional activity. H9c2 cells were transfected with control or Grx1-targeted siRNA as described in Materials and Methods, then co-transfected with NFκB-Luciferase and Renilla plasmids. NFκB-dependent transcriptional activity was determined via a standard Dual-Luciferase assay. Renilla luciferase activity served as a transfection control, and data are represented as ratios of Firefly/Renilla activity, normalized to the mean ratio observed in control cells (mean ±SEM = 33.9 ± 7.4), *p < 0.04, n = 4.