Fig. 5.

Ang-II-induced apoptosis requires SHP phosphatase. PAECs were pretreated with the following inhibitors for 20 minutes prior to Ang II (10 μM) or AT2-agonist CGP-42112A (CGP; 10 μM) treatment for 16 hours. Neutral comet assays were performed and percentages of apoptotic cells were determined. (A) PKC-inhibitor chelerythrine chloride (Chel; 1 μM) or sodium vanadate (Na₃VO₄; 20 μM), a broad-spectrum tyrosine phosphatase inhibitor; (B) PKC-inhibitor calphostin C (50 nM); (C) SHP-1/2 phosphatase inhibitor (NSC-87877; 50 μM). Data show means ± s.d. *Statistical significance from control, P<0.05, n=3. All experiments were repeated at least three times.