Fig. 9.

PAK4 is activated in prostate cancer. Endogenous PAK4 was immunoprecipitated from a matched patient pair of normal prostate cells (NP) and prostate cancer (CT) cell lines derived from the same radical prostectomy and probed for serine-474 phosphorylation. Immunoprecipitates were re-probed for total PAK4. Autoradiographs of the level of phospho-PAK4 were quantified and the signal normalised to total PAK4 levels. The results shown are the means ± s.e.m. of three independent experiments. Statistical significance compared to normal was calculated using Student's t-test; *P<0.05.