Figure 2. Effect of Betulinic acid on cell survival and apoptosis in human prostate carcinoma PC-3 cells.
A, structure of Betulinic acid. B, microphotograph of cells treated with 20 μM BetA. C, percentage of cells survived after treatment with BetA for 24, 48 and 72 h with different concentrations as demonstrated by MTT assay. Representative data Mean ± SE, n=8 which was repeated twice with similar results. D, apoptosis in the lysates from PC-3 cells treated with different concentration of BetA for 24 h, values are represented as enrichment factor described in materials and methods section. Data, Mean ± SE, n=3 significantly different from control, *p<0.05; and **p<0.001.
