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Schematic of zebra finch Cntnap2-specific riboprobes and their validation by in situ hybridization. Riboprobes were designed to hybridize to non-overlapping portions of the 3′ untranslated region (UTR) of Cntnap2, downstream of the coding sequence (CDS) (A). Both antisense (AS) riboprobes yielded identical hybridization patterns as exemplified in the hemicoronal section shown here (B). Hybridization of sense probes (S) gave no significant signal (C).
