Fig. 8.

Inverted darkfield images of emulsion autoradiography sections confirm brightfield findings of Cntnap2 hybridization in LMAN and area X. At 35d, Cntnap2 emulsion grain signals in both male (C) and female (E) LMAN appeared slightly diminished or similar compared to transcript levels in the surrounding N. Attenuated labeling in male area X (C), a song nucleus that females lack (D), stood in contrast to transcript abundance in both male (C) and female (E) St. Arrowheads point to the location of male (C) and female (E) LMAN, as identified on adjacent Nissl-stained sections (B,D). For abbreviations, see Table 1. Scale bar = 1 mm.