Figure 1. 2DE gel images of human plasma samples generated with the different sample preparation methods used during optimization.

Method A) Crude plasma - 224 spots, Method B) Aurum serum depleted plasma without desalting– 86 spots, Method C) Aurum serum depleted plasma desalted according to Khan et al. protocol– 78 spots, Method D) Aurum serum depleted plasma cleaned up using 2D-clean up kit and alkylated using iodoacetamide - 212 spots Method E) MARS depleted plasma followed by 2D-clean up and alkylated using acrylamide– 774 spots, Method F) 17 cm gel with same protocol as E– 1041 spots. Each ladder had molecular weight ranging from 10 to 220 KDa.