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. 2010 May 5;5(5):e10488. doi: 10.1371/journal.pone.0010488

Figure 3. Over-expression of Dg-C in R cells increases ommatidial size.

Figure 3

Over-expression of Dg-C was demonstrated with a concentration of anti-DGcyto antibody too low to detect wild type levels of DG (A) in third instar discs that revealed clear labeling of omatidia in transgenic flies (B). Cross sections of ommatidia (outlined) in wild type flies (C) and flies containing UAS- Dg-C/+ alone (D), elav-Gal4/+ alone (E) and elav-Gal4/UAS-Dg-C (F) showed that over-expression of Dg-C in R cells induces bigger ommatidia and expansion of R cells (F) as compared with the controls (C–E). The number of R cells remains unchanged (F). Quantification of these data shows that the ommatidia perimeters (G) are slightly but significantly increased when Dg-C is over-expressed in R cells driven by elav-Gal4 as are ommatidial areas (H). **, P<0.01. Bar, 10 µm.