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. 2010 May 5;5(5):e10506. doi: 10.1371/journal.pone.0010506

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Sethu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot analysis shows the knockdown of murine phospholipase D1 (PLD1) in BALB/c mice PBMNCs, by the use of specific siRNA for PLD1 (4 µg/mouse). (B) Western blot analysis shows the expression of murine phospholipase D2 (PLD2) in the same set of samples as indicated. Lysates probed for PLD1 and PLD2 from PBMCs were obtained from: untreated mice which served as control (Control); mice injected with siRNA-PLD1 (siRNA-PLD1); and from mice injected with scrambled siRNA (Scr-siRNA). In addition, α-tubulin was probed for loading control.