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. 2010 May 5;5(5):e10506. doi: 10.1371/journal.pone.0010506

Figure 3. TNFα-induced vascular permeability and VCAM-1 and ICAM-1 expression in peritoneal tissues is dependent on PLD1.

Figure 3

(A) Peritoneal lavage was collected, and the OD was measured, from mice: untreated following the i.p. injection of PBS (Basal); following the i.p. injection of TNFα (TNF); following the i.p. injection of TNFα in mice pretreated with the siRNA-PLD1 (TNF +siRNA-PLD1); following the i.p. injection of TNFα in mice pretreated with the scrambled siRNA (TNF +Scr-siRNA). Data showed as means ± SD of triplicate measurements from three different experiments. Student's t test p values (*p<0.05, **p<0.01). Six mice were used per treatment group per experiment. (B) VCAM-1 and ICAM-1 expression pattern using immunohistochemistry in peritoneal tissues after 2 h of TNFα administration. The panels indicate the peritoneal tissues from control mice (Basal); from mice injected with TNFα (TNF); from mice pretreated with the siRNA-PLD1 prior to TNFα administration (TNF+siRNA-PLD1); and from mice pretreated with the scrambled siRNA prior to TNFα administration (TNF+Scr-siRNA). Results shown are representative of three different experiments and of multiple sections and fields.