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. 2010 May 5;5(5):e10499. doi: 10.1371/journal.pone.0010499

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Tian et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. MTT assay of LJK-11 effects on cell growth. A549 cells were incubated with indicated concentrations of LJK-11 for 48 hours. The effect on cell growth and death was examined by MTT assay. The cell viability is expressed as a percentage of the compound-treated viable cells divided by the viable cells of the untreated control. The data are the means of triplicates ±SD. B. PARP cleavage assay of LJK-11 effects on inducing cell apoptosis. A549 cells were treated with 50 µM for indicated time periods. The cell lysates were resolved by SDS-PAGE and analyzed by Western bolt analysis using anti-PARP antibody. The cleaved PARP is indicated by the arrow. Anti-tubulin antibody was used as a protein loading control.